<2021-10-26 Tue> David biweekly meeting

projects

Project 4: Reconstitution of endocytic actin network

recent progress

  • was planning to finally set up real experimental samples, but ran into another critical snag: loss of Sac6-GFP signal during sample processing

    fluorescence_summary.png
  • did lots of troubleshooting at each step during processing, I think I narrowed down to the solution the samples are submerged in during ROI searching

    • in TBS buffer:

      MAX_4.17.4EM2.2001.jpg

    • same sample in fixative solution (what I've been using so far):

      MAX_4.17.4EM2.2_fix002.jpg
  • currently checking with Danielle if changing protocol to use buffer instead of fixative will affect the sample

next steps

weeks of <2021-10-26 Tue> to <2021-11-12 Fri>
  • prepare real experimental samples in technical replicates
  • collect correlative data
week of <2021-11-15 Mon>
  • determine whether there is a difference in membrane shape between high and low fluorescence beads
    • come up with some metric of scoring if so
weeks of <2021-11-15 Mon> to <2021-12-13 Mon>
  • if there are differences, collect triplicate data and do statistical test

Project 7: Mechanical regulation of CME by protein LLPS

recent progress

  • narrowing down issues in simulation code by reading out more intermediate calculations
  • currently found mistakes in how I wrote up volumes and curvature, so carefully going through my equations and correcting them
  • I have a pretty detailed system for reporting what's going on in the code now, so it will be obvious if any more mistakes arise

next steps

short-term
  • debug simulation code
  • add clathrin coat to model
  • add droplet to model

Date: \today

Author: Max Ferrin

Email: ferrinm@berkeley.edu

Created: 2021-10-26 Tue 13:49

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