David biweekly meeting
projects
Project 4: Reconstitution of endocytic actin network
recent progress
was planning to finally set up real experimental samples, but ran into another critical snag: loss of Sac6-GFP signal during sample processing
- did lots of troubleshooting at each step during processing, I think I narrowed
down to the solution the samples are submerged in during ROI searching
in TBS buffer:
same sample in fixative solution (what I've been using so far):